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Genel Bilgiler

Yayın Türü Hakemli Konferans/Sempozyumların Bildiri Kitaplarında Yer Alan Yayınlar ISSN Numarası
Elektronik ISSN Numarası Yayın Tarihi 2019

Detaylar

Yayın Adı Molecular identification of Phytophthora erythroseptica causing pink rot of potato in Turkey Dergi Adı 1st International Molecular Plant Protection Congress”, 44p., April 10-13, 2019, Adana, Turkey.
Dergi Cilt No
Özet Pink rot caused by Phytophthora erytroseptica was observed on potato variety Everest, grown in a 50 da field, in Konya province in 2018. About 80% of the tubers were found infected during harvest. After the infected ones discarded, still about 50% of the stored tubers rotted. Infected tubers were evident with dark brown dull lesions, and when the tubers sectioned they showed a sharp line of demarcation between healthy and diseased tissues which were a spongy texture and their colour changed from beige to pink to salmon colour after sectioned. To isolate the pathogen, the infected tissue pieces were surface sterilised with 1% sodium hypochlorite for three minutes then washed with sterile distilled water and plated on water agar (WA) and potato dextrose agar (PDA), PARP and incubated in the dark at 22±2°C for 5-7 days. Pure cultures of the pathogen were obtained by removing mycelial tips of nonseptated hyphae, growing on the isolation media and plating on Amended Grated Carrot Agar (AGCA), a medium to see sporangia, oospores and other morphological characteristics. Sporangia were observed when young culture disks taken from AGCA were incubated in sterile and nonsterile soil extract under day light for 24-48 hours. Pathogenicity of the isolate was determined by placing 5 mm dimeter culture disks of the P. erythroseptica grown on PDA into the wells drilled on surface sterilized potato tubers. The inoculated tubers were kept at 22±2°C in dark. For the molecular identification of the isolate, ITS 1 and ITS 4 regions were used. As a result of sequence after blasting the whole ITS region in NCBI web-site, the isolate showed %99 homology with many isolates of P. cryptogea and P. erythroseptica and a few isolates of P. megasperma. When the base sequences from 59 to 720 was blasted, the isolate showed 100% homology to many isolates of P. erythroseptica. This situation proves that ITS gene region alone is not sufficient to differentiate Phytophthora species from potato pink rot as in the species level. P. erythroseptica, with its homothallic nature, is a distinct species. Based on this information we also concluded that our species is also P. erythroseptica since our isolate produced abounded oospores when grown on AGCA.

Kurum İçi Yazarlar

Adı Soyadı Kısa Adı
Dr. Emel ÇAKIR Çakır E.
Tülin SARIGÜL ERTEK Ertek T S.

Kurum Dışı Yazarlar

Adı Soyadı Kısa Adı
Salih Maden Maden S.

Ekler

Dosya Adı