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Publication Type: SCI, SSCI, AHCI Index In the Articles Published in Journal ISSN No: Files
Electronic ISSN No: Release Date: 2020
Journal Name: Turkish Journal of Agricultural Science Journal No: In pres Kişiler
Summary:
Publication Type: SCI, SSCI, AHCI Index In the Articles Published in Journal ISSN No: 978-975-487-221-7 Files
Electronic ISSN No: Release Date: 2019
Journal Name: 1st International Molecular Plant Protection Congress Journal No: Kişiler
Summary: Wheat stink bug, Aelia rostrata Boheman (Hemiptera: Pentatomidae), is one of the major pests of grains in Turkey and Middle East. The pest causes significant damage, in particular, on wheat in Spring and Summer. Control of this pest is basically dependent on the chemical control. The Wheat stink bug adults feeding in the field in summer overcome the hot temperatures by migrating to mountains and entering aestivation diapause. The adults then overwinter in the top soil layers in the mountains in hibernation diapause. The lipids accumulated during feeding periods are the key resource to support the insect during hibernation and overcome the severe winter conditions. In the current study, 6 different cDNA libraries were generated: fat bodies from feeding males, feeding females, hibernating males, hibernating females, aestivating males and aestivating females. In parallel, proteomic analyses were conducted from the fat body of the corresponding samples. These analyses revealed stage-specific dramatic expression changes with particular genes restricted to hibernation and aestivation (up to a 2,600 fold change); as well as slight differences between genders. Relatively slight differences were also detected in the protein composition between feeding males and females. Interestingly, hibernating males and females were found to possess significantly higher numbers of proteins than those of feeding adults. Overall, protein composition differs slightly between genders but dramatically between feeding and non-feeding stages. Key and unique genes involved in lipid metabolism in relation to different biological stages of A. rostrata are highlighted in the current presentation.
Publication Type: Refereed conference / Proceedings of the Symposium on Location Area Publications ISSN No: 978-975-487-221-7 Files
Electronic ISSN No: Release Date: 2019
Journal Name: 1st International Molecular Plant Protection Congress Journal No: Kişiler
Summary: The midgut is a key organ in insects as it is the principal site for secretion of digestive enzymes, digestion of food and absorption of nutrients. The Sunnpest, Eurygaster maura (Hemiptera: Scutelleridae), is one of the major pests of grains in Turkey and Middle East. The pest causes significant damage, in particular, on wheat and barley in Spring and Summer. The Sunn pest has an interesting life cycle with one generation per year. Nymphs hatch from an egg in spring, become adults, then migrate into the mountains and overcome the hot temperatures by entering aestivation diapause. The adults hibernate in the soil under bushes and oak trees for around 6 months, emerge and migrate back to field by spring, where they mate then die. It is important to understand the physiological and biochemical changes in midgut in feeding and non-feeding periods as these changes are the key factors for survival, overcoming the hibernation, and influence the feeding damage on the plants. In the current study, we examined the midgut tissues from feeding and hibernating adults using a proteomic, LC/MS-MS approach. The peptides obtained through proteomics were scanned in a midgut specific cDNA library generated from the feeding adults. In each sample, midgut samples from females and males were mixed equally in order to eliminate the gender-based biochemical differences. The cDNA library from the feeding adults generated 16,231 contigs. The midguts of feeding and hibernating adults were found to possess 702 and 623 proteins, respectively. The dominant functional proteins (excluding ribosomal, protein trafficking and other cellular proteins) were digestive enzymes, cellular and humoral defense responses, detoxification, lipid metabolism, carbohydrate metabolism, calcium homeostasis, circadian clock and cold tolerance. Digestive enzymes were predominant in the feeding stage, while proteins related to cellular and humoral defense, lipid and carbohydrate metabolism, calcium homeostasis, circadian clock and cold tolerance were predominant in the hibernating stage. Key words: Midgut, proteomics, feeding, hibernation, sunnpest, Eurygaster maura
Publication Type: SCI, SSCI, AHCI Index In the Articles Published in Journal ISSN No: Files
Electronic ISSN No: Release Date: 2019
Journal Name: Applied Ecology and Environmental Research Journal No: 17 (3), 5987-5994 Kişiler
Summary:
Publication Type: Refereed conference / Proceedings of the Symposium on Location Area Publications ISSN No: Files
Electronic ISSN No: Release Date: 2019
Journal Name: 1st International Molecular Plant Protection Congress, April 10-13 2019, Adana TURKEY Journal No: Kişiler
Summary: The tomato leafminer, Tuta absoluta (Lepidoptera), a destructive and invasive pest of tomatoes, is mainly controlled through the use of chemical insecticides. Altough diamide insectides, such as chlorantraniliprole with novel mode of action, have newly been registered, they have been intensively used because of their high efficacy level at low rates. However, their intensive use has led to the development of resistance. High levels of chlorantraniliprole resistance have recently been described in T. absoluta populations in Greece, Italy, Brazil, Spain and Isreal. In the present study, biological and molecular methods were used to determine the susceptibility of one field collected T. absoluta strain from Turkey, Antalya to chlorantraniliprole. High level of resistance was observed exhibiting (LC50: 813.522 [478.596 – 1807.186, in ppm]). To investigate whether chlorantraniliprole resistance was mediated by point mutation on ryanodine receptor (RyR) in T. absoluta, we sequenced partial RyR gene containing mutation sites recently shown to confer chlorantraniliprole resistance. This revealed the prescence of G4903E and I4746M mutations and supports the suggestion that control failures in Turkey especially in Antalya, in part, due to mutations on RyR target-site. Involment of detoxification enzymes should also be investigated. Areas used chlorantraniliprole against T. absoluta should be monitored to minimize the risk of rapid selection for high levels of resistance and to surpress the further spread of resistant populations, insecticide resistant management strategies should be adapted.
Publication Type: Refereed conference / Proceedings of the Symposium on Location Area Publications ISSN No: 5 Files
Electronic ISSN No: Release Date: 2019
Journal Name: 1st International Molecular Plant Protection Congress Adana TURKEY - April 10-13 2019 Journal No: Kişiler
Summary:
Publication Type: Refereed conference / Proceedings of the Symposium on Location Area Publications ISSN No: Files
Electronic ISSN No: Release Date: 2019
Journal Name: 1st International Molecular Plant Protection Congress Journal No: Kişiler
Summary: Recent have studies identified Cylindrocladiella species as grapevine pathogens and associated black-foot diesase. Isolates morphologically identified as Cylindrocladiella parva were isolated from characteristic black foot symptoms on a grapevine (Vitis vinifera) from Central Anatolia Region, Ankara province. On PDA the isolates initially produced cottony, white mycelia that turned grayish cream the initially tawny colony undersides becoming dark brown with age. Conidia (0 to 1 septate; 16.3 to 17.1 × 2.2 to 2.7 µm) and abundant chlamydospores were produced (Lombard et al. 2012 and Erper et al. 2013). The ß-tubulin gene fragments (primers T1 and Bt2b) of two isolates were sequenced (Genbank Accession No. KY496705, KY496706) (Coller et al. 2005). Koch's postulates were completed on five shoots inoculated with C. parva isolate. Inoculations were made on green shoots of V. vinifera cv. Sultana T. There were six replicate shoots per isolate, with an equal number of controls, and the experiment was repeated once. Shoots were wounded 5 cm from the apical and using a 4 mm diameter cork borer to remove the cortex tissue. Colonised mycelial plugs from 2-week old cultures. were inserted into the wounds and sealed with Parafilm. Control treatment were used non-colonised PDA plugs. Inoculated shoots had necrotic lesions and re-isolations were made from lesions. No lesions developed in the control shoots. To our knowledge, the present study is the first report confirming the presence of C. parva associated with black foot disease of grapevine in Turkey.
Publication Type: Refereed conference / Proceedings of the Symposium on Location Area Publications ISSN No: 978-975-487-221-7 Files
Electronic ISSN No: Release Date: 2019
Journal Name: 1st International Molecular Plant Protection Congress Journal No: Kişiler
Summary: Insects are one of the leading factors that cause of product losses in stored grains. The most important control form against these pests is fumigation. Fumigant phosphine is used extensively in fumigation worldwide and in Turkey. In the current study, phosphine resistance of the grain weevil Sitophilus granarius and the rice weevil S.oryzae populations were tested. Resistance ratios of resistant populations were determined by LC50 value. 21 of the 26 populations of S. granarius were susceptible, the five populations were resistant and the maximum resistance was 5.26 folds. 17 populations of S. oryzae were susceptible, 11 populations were resistant and maximum resistance was 200.54 folds. Some molecular studies were carried out to determine the resistance variants using the resistant and susceptible individuals of the pests. As a result of studies on the rph2 gene encoding the enzyme Dihydrolipoamide dehydrogenase (DLD) which causes phosphine resistance in Sitophilus spp., no variant was determined in S. granarius resistant individuals but P48S (Proline>Serine) and K141E (Lysine>Glutamic acid) variants were determined on the DLD gene in S. oryzae. These two variants were first time detected in S. oryzae.
Publication Type: Refereed conference / Proceedings of the Symposium on Location Area Publications ISSN No: 978-975-487-221-7 Files
Electronic ISSN No: Release Date: 2019
Journal Name: 1st International Molecular Plant Protection Congress, April 10-13 2019, Adana TURKEY Journal No: Kişiler
Summary: Insects are one of the leading factors that cause of product losses in stored grains. The most important control form against these pests is fumigation. Fumigant phosphine is used extensively in fumigation worldwide and in Turkey. In the current study, phosphine resistance of the grain weevil Sitophilus granarius and the rice weevil S.oryzae populations were tested. Resistance ratios of resistant populations were determined by LC50 value. 21 of the 26 populations of S. granarius were susceptible, the five populations were resistant and the maximum resistance was 5.26 folds. 17 populations of S. oryzae were susceptible, 11 populations were resistant and maximum resistance was 200.54 folds. Some molecular studies were carried out to determine the resistance variants using the resistant and susceptible individuals of the pests. As a result of studies on the rph2 gene encoding the enzyme Dihydrolipoamide dehydrogenase (DLD) which causes phosphine resistance in Sitophilus spp., no variant was determined in S. granarius resistant individuals but P48S (Proline>Serine) and K141E (Lysine>Glutamic acid) variants were determined on the DLD gene in S. oryzae. These two variants were first time detected in S. oryzae.
Publication Type: Articles published in other Journals ISSN No: Files
Electronic ISSN No: Release Date: 2019
Journal Name: Atatürk University Journal of The Agricultural Faculty Journal No: Kişiler
Summary:
Publication Type: Refereed conference / Proceedings of the Symposium on Location Area Publications ISSN No: Files
Electronic ISSN No: Release Date: 2019
Journal Name: 1th International Congress on Sustainable Agriculture and Technology, Gaziantep. 01-02 April 2019. Journal No: Kişiler
Summary:
Publication Type: Articles published in other Journals ISSN No: Files
Electronic ISSN No: doi:10.1007/s41348-019-00206-y Release Date: 2019
Journal Name: Journal of Plant Diseases and Protection Journal No: doi:10.1007/s41348-019-00206-y Kişiler
Summary: In 2015 and 2017, potato storages were surveyed for the prevalence of atypical potato tuber rots in Nevşehir and Isparta Provinces of Turkey. About 4–5 months after harvesting, 80% of the potatoes cv. Melody, that were stored at 4–5 °C and 65–75% relative humidity, were affected by the disease. The symptoms of the disease were similar to gangrene caused by Boeremia exigua var. foveata including superficial, dark lesions or dark brown, sunken lesions that have a sharp border with healthy tissue on tubers. A fungus was consistently isolated from necrotic tuber tissues. The pathogen was identified as Colletotrichum coccodes (Wallr.) Hughes based on morphological characteristics and the analysis of sequence of the internal transcribed spacer region of the rDNA. Pathogenicity was tested by tuber inoculation with mycelial agar discs. After 4–6 weeks, irregular and dark brown sunken lesions developed on potato tubers, while control tubers remained symptomless. This is the first report of C. coccodes that caused extraordinary symptoms on potato tubers in Turkey.
Publication Type: Refereed conference / Proceedings of the Symposium on Location Area Publications ISSN No: Files
Electronic ISSN No: Release Date: 2019
Journal Name: 1st International Molecular Plant Protection Congress”, April 10-13, 2019, Adana, Turkey. Journal No: Kişiler
Summary:
Publication Type: Refereed conference / Proceedings of the Symposium on Location Area Publications ISSN No: Files
Electronic ISSN No: Release Date: 2019
Journal Name: 1st International Molecular Plant Protection Congress”, April 10-13, 2019, Adana, Turkey. Journal No: Kişiler
Summary:
Publication Type: Refereed conference / Proceedings of the Symposium on Location Area Publications ISSN No: Files
Electronic ISSN No: Release Date: 2019
Journal Name: 1st International Molecular Plant Protection Congress”, April 10-13, 2019, Adana, Turkey. Journal No: Kişiler
Summary:
Publication Type: Refereed conference / Proceedings of the Symposium on Location Area Publications ISSN No: Files
Electronic ISSN No: Release Date: 2019
Journal Name: 1st International Molecular Plant Protection Congress”, April 10-13, 2019, Adana, Turkey. Journal No: Kişiler
Summary:
Publication Type: SCI, SSCI, AHCI Index In the Articles Published in Journal ISSN No: Files
Electronic ISSN No: Release Date: 2019
Journal Name: 1st International Molecular Plant Protection Congress”, April 10-13, 2019, Adana, Turkey. Journal No: Kişiler
Summary:
Publication Type: Articles published in other Journals ISSN No: Files
Electronic ISSN No: Release Date: 2019
Journal Name: Entomofauna Journal No: 40/1: .. Kişiler
Summary: Faunistic and systematic studies on Miridae of Turkey are reviewed and the distribution and biogeography of the Turkish Miridae fauna is analyzed. In this study, two species in one genus of Bryocorinae, eight species in two genera of Deraeocorinae, 62 species in 30 genera of Mirinae, 18 species in 12 genera of Orthotylinae and 32 species in 20 genera of Phylinae are recorded. In total, 123 species belonging to 65 genera of five subfamilies of the family Miridae are recorded from Turkey. In this paper, the publications on the Miridae in Turkey were reviewed and the biogeography of the Turkish fauna of Miridae have been analyzed. Species composition, diversity and proportion of endemism varies considerably between the biogeographic subregions of the country
Publication Type: SCI, SSCI, AHCI Index In the Articles Published in Journal ISSN No: Files
Electronic ISSN No: Release Date: 2019
Journal Name: Fresenius Environmental Bulletin Journal No: 28 (4), 2916-2922 Kişiler
Summary:
Publication Type: Refereed conference / Proceedings of the Symposium on Location Area Publications ISSN No: 978-975-487-221-7 Files
Electronic ISSN No: Release Date: 2019
Journal Name: 1st International Molecular Plant Protection Congress. Program and Abstract Book. 91 p. Journal No: April 10-13 2019 Adana, Turkey. Kişiler
Summary: The only phytoplasma disease known in Turkey on potato is ‘Stolbur disease’. The causal agent of this disease, Candidatus Phytoplasma solani (CPs), is a quarantine organism and has zero tolerance for field inspection at seed potato production. CPs is transmitted from infected plants to healthy ones via Hyalesthes obseletus that is known to be the main vector in Turkey. During the 2013-2014 vegetation period, the survey of 248 localities in Bolu, Kırşehir, Sivas, and Yozgat provinces revealed the composition of Hemipteran fauna in potato fields. The collecting of insects performed to these provinces between June and August at the flowering period of potatoes using sweep nets. It has been determined that 13 species belonging to four families. Nine species from the family of Cicadellidae, two species from Delphacidae, one species from Cixiidae and Tettigometridae were identified morphologically. These are Asymmetrasca decedens, Circulifer haematoceps, Empoasca decipens, Euscelis incisus, Hyalesthes obsoletus, Laodelphax striatellus, Macrosteles laevis, Neoaliturus fenestratus, Psammotettix cephalotes, P. striatus, P. provincialis, Tettigometra sulphurea and Toya propinqua. There is no any positive result the presence of CPs in insects after nested PCR using P1/P7 and R16F2n/R16R2 primers. Two known vectors of CPs in Turkey, H. obsoletus and M. laevis, were found in this study, however, there is a need for studies on other species, which may be a potential vector for CPs.