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Genel Bilgiler

Yayın Türü SCI, SSCI, AHCI İndekslerine Giren Dergilerde Yayınlanan Makaleler ISSN Numarası 0929-1873
Elektronik ISSN Numarası 1573-8469 Yayın Tarihi 2018

Detaylar

Yayın Adı Performance of diagnostic tests for the detection and identification of Pseudomonas syringae pv. actinidiae (Psa) from woody samples Dergi Adı European Journal of Plant Pathology
Dergi Cilt No 152 (3) 657-676
Özet The aim of this study was to characterise the performance of new molecular methods for the detection and identification of Pseudomonas syringae pv. actinidiae (Psa) and to provide validation data in comparison to the assays mentioned in official diagnostic protocols and being currently used. Eleven molecular tests for the Psa detection were compared in an inter-laboratory comparison where each laboratory had to analyse the same panel of samples consisting of thirteen Psa-spiked kiwifruit wood extracts. Laboratories had to perform also isolation from the wood extracts. Data from this interlaboratory test performance study (TPS) was statistically analysed to assess the performance of each method. In order to provide complete validation data, both for detection and identification, this TPS was supplemented by a further study of identification from pure culture of phylogenetically closely related Pseudomonas spp., Psa, and bacterial strains associated with kiwifruit. The results of both these studies showed that simplex-PCRs gave good results, whereas duplex-PCR and real-time PCR were the most reliable tools for detection and identification of Psa. Nested and multiplex-PCR gave false-positive results. The use of the most reliable detection test is suggested for routine analyses, but when Psa-free status needs to be accurately assessed, it is recommended that at least two detection tests are used. This work provides a wide comparison of the available diagnostic methods, giving new information for a possible revision of the official diagnostic protocols (e.g. European and Mediterranean Plant Protection Organization (EPPO) protocol PM7/120 for the detection of Psa).

Kurum İçi Yazarlar

Adı Soyadı Kısa Adı
Dr. Aynur KARAHAN Karahan A.

Kurum Dışı Yazarlar

Adı Soyadı Kısa Adı
Stefania Loreti Loreti.S
Amandine Cunty Cunty.A
Nicoletta Pucci Pucci.N
Aude Chabirand Chabirand.A
Emilio Stefani Stefani. E
Adela Abelleira Abelleira. A
Giorgio M. Balestra Balestra G.M.
Deirdre A. Cornish Cornish D.A.
Francesca Gaffuri Gaffuri F.
Davide Giovanardi Giovanardi D.
Richard A. Gottsberger Gottsberger R.A.
Maria Holeva Holeva M.
Charikleia D. Karafla Karafla C.D.
Angelo Mazzaglia Mazzaglia A.
Robert Taylor Taylor R.
Leonor Cruz Cruz L.
Maria M. Lopez Lopez M.M.
Joel L. Vanneste Vanneste J.L.
Françoise Poliakoff Poliakoff F.

Ekler

Dosya Adı