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Yayın Türü SCI, SSCI, AHCI İndekslerine Giren Dergilerde Yayınlanan Makaleler ISSN Numarası
Elektronik ISSN Numarası Yayın Tarihi 2009


Yayın Adı First report of Clavibacter michiganensis subsp. sepedonicus causing bacterial ring rot of potato in Turkey Dergi Adı Plant Pathology
Dergi Cilt No Volume 58, Issue 4, Page 794
Özet During the 2007 seed potato monitoring programme in Turkey, 336 tuber samples from Kayseri province were tested for the presence of ring rot and brown rot diseases according to the EC Directives 93/85/EEC (Anon., 1993) and 98/57/EC (Anon., 1998). Three samples gave a positive immunofluorescence test (IF) result for the ring rot pathogen, Clavibacter michiganensis subsp. sepedonicus (Spieckermann & Kotthoff) Davis. et al.(Cms). Tuber heel end extracts were plated onto NCP-88 medium. Creamy-white, smooth, mucoid-fluidal colonies were selected and purified. External symptoms were observed on tubers of all three samples (two of cv. Soleia and one of cv. Safrane) as sunken and cracked areas (Fig. 1). When tubers were cut transversely, typical symptoms were seen through the vascular ring of tubers as a brown cheesy decay. Bacterial ooze was expressed when tubers were squeezed (Fig. 2). A positive IF result was obtained directly from the ooze. Isolates hydrolysed aesculin and produced catalase. They did not produce acid from glycerol, lactose, rhamnose and salicin. They gave negative results for oxidase activity, growth at 37°C, urease activity, starch hydrolysis, tolerance of 7 % NaCl, indole production and gelatine liquefaction. Suspensions of pure colonies in water (c. 109 cfu per ml from each of the three samples were used for real-time PCR (Schaad et al. ,1999), with Ct values of 16.53, 16.21 and 32.2. Suspensions (106 cfu/ml) were injected into stems of ten Solanum melongena plants (cv. Black Beauty) at leaf stage 3. Control plants were inoculated with sterile water. Plants were incubated at 21°C and 70-80 % humidity. First symptoms were observed as dark green areas on leaves after ten days followed by wilting and necrosis (Figure 3). Cms was re-isolated and identified. An intensive survey in 1988-1989 had shown that the potato growing areas of Turkey were free of the ring rot pathogen (Benlioğlu et al., 1991). This is the first report of Cms in Turkey. Strict control measures have been taken in contaminated and potentially contaminated fields since Cms is a regulated quarantine organism in Turkey.

Kurum İçi Yazarlar

Adı Soyadı Kısa Adı
Şenol ALTUNDAĞ Altundağ Ş.
Dr. Aynur KARAHAN Karahan A.

Kurum Dışı Yazarlar

Adı Soyadı Kısa Adı
Aliosman Kılınç AO.Kılınç
Meriç ÖZAKMAN M.Ozakman


Dosya Adı